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产品目录

AsuC2I内切酶图片
产品货号:
GS2186
中文名称:
AsuC2I内切酶
英文名称:
AsuC2I
产品规格:
1kU
发货周期:
1~3天
产品价格:
询价
储存条件:冷冻(-20℃)

技术规格

Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sitesCC↑SGG
来源Actinobacillus suis CA
Assayed onLambda DNA
Optimal bufferY(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.)
Optimal temperature37℃
Storage conditions10 mM Tris-HCl(pH 7.5);250 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;50%glycerol;Store at -20℃.
LigationsAfter 5-fold overdigestion less than 10%of the DNA fragments can be ligated
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1μgof Lambda DNA with 50 u.a.of enzyme for 16hoursat 37℃.
Methlation effectMethylation sensitivity not tested
Thermal inactivationenzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible EndsBcnI,BpuMI,CauII,EcoHI^,NciI

相关搜索:AsuC2I内切酶AsuC2I限制性内切酶
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